Micronucleus yield and colorimetric test as indicators of damage in patients' lymphocytes after

C. Catena, D. Conti, G. Trenta, E. Righi, F. Breuer, F. Fiore Melacrinis, T. Montesano, G. Ventroni, G. Ronga

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Abstract

To estimate the absorbed dose received by patients who underwent131I therapy, a modified compartmental model of the International Commission on Radiological Protection (ICRP) was used. The activity in plasma and micronucleus (MN) frequency (MN test) were measured before and after therapy. To evaluate whether a correlation exists between lymphocytes and absorbed dose, a colorimetric test, based on the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT test), was used. Methods: Twenty patients who underwent131I therapy were studied. Activity was measured in plasma, and isolated lymphocytes were collected to perform the MN and MTT tests. Results: The mean MN frequency observed in unexposed patient lymphocytes was comparable with that of healthy subjects.131I therapy induces a small increase in MN, and a good correlation with the bone marrow absorbed dose was obtained (P = 0.040). A consistent decrease in phytostimulation observed after therapy (MTT test) correlated significantly with bone marrow absorbed dose (P = 0.0085). Conclusion: The MTT test appears to be more reliable than the MN test for evaluating lymphocyte damage induced by131I therapy.
Original languageEnglish
Pages (from-to)1522 - 1524
Number of pages3
JournalJournal of Nuclear Medicine
Volume41
Issue number9
Publication statusPublished - 2000
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Cite this

Catena, C., Conti, D., Trenta, G., Righi, E., Breuer, F., Fiore Melacrinis, F., ... Ronga, G. (2000). Micronucleus yield and colorimetric test as indicators of damage in patients' lymphocytes after. Journal of Nuclear Medicine, 41(9), 1522 - 1524.