Multiple presentation of Scfv800E6 on silica nanospheres enhances targeting efficiency Toward HER-2 Receptor in Breast Cancer Cells

Serena Mazzucchelli, Paolo Verderio, Silvia Sommaruga, Miriam Colombo, Agnese Salvadè, Fabio Corsi, Patrizia Galeffi, Paolo Tortora, Davide Prosperi

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9 Citations (Scopus)

Abstract

Spherical silica nanoparticles (SNP) have been synthesized and functionalized with anti-HER-2 scFv800E6 antibody by both localized histidine-tag recognition, leading to an oriented protein ligation, and glutaraldehyde cross-linking, exploiting a statistical reactivity of lysine amine groups in the primary sequence of the molecule. The targeting efficiency of nanocomplexes in comparison with free scFv was evaluated by flow cytometry using a HER-2 antigen-positive MCF-7 breast cancer cell line, exhibiting a 4-fold increase in scFv binding efficacy, close to the affinity of intact anti-HER-2 monoclonal antibody, which suggests the effectiveness of presenting multiple scFv molecules on nanoparticles in improving antigen recognition. Unexpectedly, the conjugation method did not affect the binding efficacy of scFv, suggesting a structural role of lysines in the scFv molecule. Confocal laser scanning microscopy confirmed the binding of nanocomplexes to HER-2 and also provided evidence of their localization at the cell surface. © 2011 American Chemical Society.
Original languageEnglish
Pages (from-to)2296 - 2303
Number of pages8
JournalBioconjugate Chemistry
Volume22
Issue number11
DOIs
Publication statusPublished - 16 Nov 2011
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Mazzucchelli, S., Verderio, P., Sommaruga, S., Colombo, M., Salvadè, A., Corsi, F., ... Prosperi, D. (2011). Multiple presentation of Scfv800E6 on silica nanospheres enhances targeting efficiency Toward HER-2 Receptor in Breast Cancer Cells. Bioconjugate Chemistry, 22(11), 2296 - 2303. https://doi.org/10.1021/bc200352x