Optimizing environmental factors for large-scale multiplication of chrysanthemum (Chrysanthemum grandiflorum) in balloon-type bioreactor culture

G. Sivakumar, S.J. Kim, E.J. Hahn, K.Y. Paek

Research output: Contribution to journalArticle

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We have developed optimum culture conditions for the large-scale propagation of chrysanthemum in balloon-type bioreactors to achieve vigorous growth and quality. The effects of NH4+/NO3-ratio, air volume, air temperature, photosynthetic photo flux, and an inoculation density on the growth and quality of plantlets were investigated. The best production conditions were an NH4+:NO3-ratio of 20:40 mM, air exchange of 0.1 vvm min-1, air temperature 25°C, photosynthetic photo flux (PPF) at 100 μmol m-2s-1, and an inoculation density of 40 nodes Chrysanthemum grandiflorum. Under each of these conditions, the maximum growth rate reached 279.0, 260.0, 20.0, 23.3. and 94.5 (g-fresh weight per plantlet d-1), respectively, at 12 wk of culture. These results specify the key environmental factors that can be regulated to improve the quality and quantity of flowers and increase yield in large-scale bioreactor cultures of chrysanthemum. © 2005 Society for In Vitro Biology.
Original languageEnglish
Pages (from-to)822 - 825
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Plant
Issue number6
Publication statusPublished - Nov 2005
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Plant Science
  • Developmental Biology
  • Cell Biology

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