Organogenesis and embryogenesis from callus cultures of azadirachta excelsa

Germina Giagnacovo, GabrieIla Pasqua, Barbara Monacelli, Stephen Van der Esch, Oliviero Maccioni, Fabio Vitali

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Abstract

Callus production from leaf explants of Azadirachta excelsa (Jack) Jacobs was favoured by Murashige and Skoog medium supplemented with 4 mg l-1indole-3-butyric acid (IBA) and 1 mg 1-16-benzyladenine (BAP). Increasing the concentration of BAP to 2 mg l-1induced shoot regeneration. Adding polyvinylpyrrolidone (PVP) to the medium resulted in a significantly increased number of shoots. Transfer onto medium containing 0.5 mg l-1BAP, 0.4 mg l-1gibberellic acid (GA3) and 2% sucrose stimulated elongation of the intemodes; subsequent transfer onto medium containing 1 mg l-1IBA induced root formation. The histological analysis demonstrated that organogenesis and embryogenesis occurred in the same callus. However, shoots originated inside the callus mass, whereas the embryos originated on the surface. Given that the embryos did not develop beyond the globular or heart-shaped stage, we concluded, that the plants regenerated from callus were derived only from organogenesis. © 2001 Taylor & Francis Group, LLC.
Original languageEnglish
Pages (from-to)13 - 18
Number of pages6
JournalPlant Biosystems
Volume135
Issue number1
DOIs
Publication statusPublished - 1 Jan 2001
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

Giagnacovo, G., Pasqua, G., Monacelli, B., Van der Esch, S., Maccioni, O., & Vitali, F. (2001). Organogenesis and embryogenesis from callus cultures of azadirachta excelsa. Plant Biosystems, 135(1), 13 - 18. https://doi.org/10.1080/11263500112331350590