Phenotypic and genotypic characteristics of new euploid-diploid lymphoblastoid B cell lines EBV

Luisa Bertolini, Maria Luisa Aebischer, Franco Ameglio, Antonio Angeloni, Isabella Delaroche, Alberto Faggioni, Alberto Fruscalzo, Giovanni Gorini, Annalucia Serafino, Giuseppe Starace, Antonio Tabilio

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Abstract

The present study describes the phenotypic and genotypic features of seven individual growth transformed, euploid-diploid EBV+human B cell lines arisen spontaneously in vitro. The lines, obtained under general and standard culture conditions (un-manipulated), from seven individual bone marrow samples of 18 healthy young adults, Caucasian, of both sexes, display many traits of normal B cells and represent a mixture of EBV infected latently (latency type III) and producer cells (5-16% VCA+by immunofluorescence) releasing seven individual different viral strains [Fruscalzo et al., 2001. DNA sequence heterogeneity within the Epstein-Barr virus family of repeats in the latent origin of replication. Gene 265, 165-173] similar to the B95-8 genotype as shown by results of Southern blot of BamHI-digested DNA fragment. These tests were planned to characterize more fully this panel of new bone marrow cell lines sharing normal B cell traits. © 2005 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)91 - 100
Number of pages10
JournalJournal of Virological Methods
Volume126
Issue number1-2
DOIs
Publication statusPublished - 2005
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Bertolini, L., Aebischer, M. L., Ameglio, F., Angeloni, A., Delaroche, I., Faggioni, A., ... Tabilio, A. (2005). Phenotypic and genotypic characteristics of new euploid-diploid lymphoblastoid B cell lines EBV. Journal of Virological Methods, 126(1-2), 91 - 100. https://doi.org/10.1016/j.jviromet.2005.01.029