Pseudomonas putida based amperometric biosensors for 2,4-D detection

Dilek Odaci, Mustafa Kemal Sezgintürk, Suna Timur, Nurdan Pazarlioǧlu, Roberto Pilloton, Erhan Dinçkaya, Azmi Telefoncu

Research output: Contribution to journalArticle

8 Citations (Scopus)


Amperometric biosensors using Pseudomonas putida cells as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). After the adaptation process of Pseudomonas putida to 2,4-D, cells were immobilized onto the screen printed graphite electrodes (SPG) as well as Clark oxygen probe by gelatin and glutaraldehyde. Optimum pH, temperature, and stability of the biosensor were investigated. Substrate specificities for various phenolic compounds were also searched. In repeatability studies, variation coefficients and standard deviations for both type of systems were calculated; SPG and Clark electrodes were calculated and results are given as a comparison of two systems. Finally, the biosensors were applied to 2,4-D determination in a real herbicide sample. Copyright © Taylor & Francis Group, LLC.
Original languageEnglish
Pages (from-to)11 - 19
Number of pages9
JournalPreparative Biochemistry and Biotechnology
Issue number1
Publication statusPublished - Jan 2009
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biotechnology

Cite this

Odaci, D., Sezgintürk, M. K., Timur, S., Pazarlioǧlu, N., Pilloton, R., Dinçkaya, E., & Telefoncu, A. (2009). Pseudomonas putida based amperometric biosensors for 2,4-D detection. Preparative Biochemistry and Biotechnology, 39(1), 11 - 19.