Raman spectroscopy-based approach to detect aging-related oxidative damage in the mouse oocyte

Luisa Bogliolo, Ombretta Murrone, Giovanna Di Emidio, Massimo Piccinini, Federica Ariu, Sergio Ledda, Carla Tatone

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23 Citations (Scopus)

Abstract

Purpose: Detection of chemical modifications induced by aging-related oxidative damage in mouse metaphase II (MII) oocytes by Raman microspectroscopy. Methods: CD-1 mice at the age of 4-8 weeks (young mice) and 48-52 weeks (old mice), were superovulated and oocytes at metaphase II stage were recovered from oviducts. MII oocytes from young animals were divided into three groups: A) young oocytes, processed immediately after collection; B) in vitro aged oocytes, cultured in vitro for 10 h before processing; C) oxidative-stressed oocytes, exposed to 10 mM hydrogen peroxide for 2 min before processing. Oocytes from reproductively old mice were referred to as old oocytes (D). All the oocytes were analyzed by confocal Raman microspectroscopy. The spectra were statistically analyzed using Principal Component Analysis (PCA). Results: PCA evidenced that spectra from young oocytes (A) were clearly distinguishable from those obtained from in vitro-aged, oxidative-damaged and old oocytes (B, C, D) and presented significant differences in the bands attributable to lipid components (C = C stretching, 1,659 cm-1; CH2bending, 1,450 cm-1; CH3deformation,1,345 cm-1; OH bending, C-N stretching, 1,211 cm-1) and protein components (amide I band,1,659 cm-1; CH2bending modes and CH3deformation, 1,450 cm-1; C-N and C-C stretching vibrations, 1,132 cm-1; phenylalanine's vibration, 1,035 cm-1) Conclusions: Raman spectroscopy is a valuable non-invasive tool for the identification of biochemical markers of oxidative damage and could represent a highly informative method of investigation to evaluate the oocyte quality. © 2013 Springer Science+Business Media New York.
Original languageEnglish
Pages (from-to)877 - 882
Number of pages6
JournalJournal of Assisted Reproduction and Genetics
Volume30
Issue number7
DOIs
Publication statusPublished - Jul 2013
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)

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