Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations

Manhai Long, Alessandra Stronati, Davide Bizzaro, Tanja Krüger, Gian-Carlo Manicardi, Philip S. Hjelmborg, Marcello Spanò, Alexander Giwercman, Gunnar Toft, Jens Peter Bonde, Eva C. Bonefeld-Jorgensen

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Abstract

Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon receptor (AhR) activities, and the sperm DNA damage and sperm apoptotic markers of 262 adult males (54 Inuits from Greenland, 69 from Warsaw (Poland), 81 from Sweden, and 58 from Kharkiv (Ukraine)) exposed to different levels of POPs. Xenobiotic-induced receptor activities were determined by receptor-mediated luciferase reporter gene expression. Sperm DNA damage was measured using terminal deoxynucleotidyl transferase-driven dUTP nick labeling assay (TUNEL) and pro- (Fas) and anti-apoptotic (Bcl-xL) markers were determined by immune methods. Different features of xenobiotic-induced receptor activity in serum and sperm DNA fragmentation and apoptotic markers existed between the Inuits and the European Caucasians. Negative correlations between xenobiotic-induced receptor activities and DNA damage were found for Inuits having relatively lower xenoestrogenic, lower dioxin-like activity, and lower sperm DNA damage, but higher xenoandrogenic activity. In contrast, in the European groups, xenobiotic-induced receptor activities were found to be positively correlated with the DNA damage. Further research must elucidate whether altered receptor activities in concerted action with genetic and/or nutrient factors may have protecting effect on sperm DNA damage of the Inuit population. © 2007 Society for Reproduction and Fertility.
Original languageEnglish
Pages (from-to)517 - 530
Number of pages14
JournalReproduction
Volume133
Issue number2
DOIs
Publication statusPublished - Feb 2007
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Obstetrics and Gynaecology
  • Cell Biology

Cite this

Long, M., Stronati, A., Bizzaro, D., Krüger, T., Manicardi, G-C., Hjelmborg, P. S., ... Bonefeld-Jorgensen, E. C. (2007). Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations. Reproduction, 133(2), 517 - 530. https://doi.org/10.1530/REP-06-0195