Neuroblastoma cells can undergo neural differentiation upon treatment with a variety of chemical inducers and growth factors. During this process, many cell cycle-related genes are downregulated while differentiation- specific genes are triggered. The retinoblastoma family proteins, pRb, p107, and pRb2/p130, are involved in transcriptional repression of proliferation genes, mainly through their interaction with the E2F transcription factors. We report that pRb2/p130 expression levels increased during differentiation of neuroblastoma cell line LAN-5. On the other hand, both pRb and p107 decreased and underwent progressive dephosphorylation at late differentiation times. The expression of B-myb and c-myb, two targets of the retinoblastoma family proteins, were downregulated in association with the increase of pRb2/p130, which was detected as the major component of the complex with E2F on the E2F site of the B-myb promoter in differentiated cells. Interestingly, E2F4, a preferential partner of p107 and pRb2/p130, was upregulated and underwent changes in cellular localization during differentiation. In conclusion, our data suggest a major role of pRb2/p130 in the regulation of B-myb promoter during neural differentiation despite the importance of cofactors in modulating the function of the retinoblastoma family proteins.
|Pages (from-to)||297 - 303|
|Number of pages||7|
|Journal||Journal of Cellular Biochemistry|
|Publication status||Published - 1 Dec 1997|
All Science Journal Classification (ASJC) codes
- Cell Biology
Raschellà, G., Tanno, B., Bonetto, F., Amendola, R., Battista, T., De Luca, A., ... Paggi, M. G. (1997). Retinoblastoma-related protein pRb2/p130 and its binding to the B-myb promoter increase during human neuroblastoma differentiation. Journal of Cellular Biochemistry, 67(3), 297 - 303. https://doi.org/10.1002/(SICI)1097-4644(19971201)67:3<297::AID-JCB2>3.0.CO;2-R