Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)6-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor μ-array. © 2004 Elsevier B.V. All rights reserved.
All Science Journal Classification (ASJC) codes
- Physical and Theoretical Chemistry
Maly, J., Di Meo, C., De Francesco, M., Masci, A., Masojidek, J., Sugiura, M., ... Pilloton, R. (2004). Reversible immobilization of engineered molecules by Ni-NTA chelators. Bioelectrochemistry, 63(1-2), 271 - 275. https://doi.org/10.1016/j.bioelechem.2003.10.024