Reversible immobilization of engineered molecules by Ni-NTA chelators

J. Maly, C. Di Meo, M. De Francesco, A. Masci, J. Masojidek, M. Sugiura, A. Volpe, R. Pilloton

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Abstract

Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)6-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor μ-array. © 2004 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)271 - 275
Number of pages5
JournalBioelectrochemistry
Volume63
Issue number1-2
DOIs
Publication statusPublished - Jun 2004

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

Cite this

Maly, J., Di Meo, C., De Francesco, M., Masci, A., Masojidek, J., Sugiura, M., ... Pilloton, R. (2004). Reversible immobilization of engineered molecules by Ni-NTA chelators. Bioelectrochemistry, 63(1-2), 271 - 275. https://doi.org/10.1016/j.bioelechem.2003.10.024