Screen printed graphite biosensors based on bacterial cells

Suna Timur, Livia Della Seta, Nurdan Pazarlioǧlu, Roberto Pilloton, Azmi Telefoncu

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28 Citations (Scopus)

Abstract

A microbial biosensor was developed for the determination of phenolic compounds and the measurement was based on oxygen consumption in relation to analyte oxidation. Induced cells of Pseudomonas putida DSM 50026 were immobilised on the surface of SPG electrodes covered with cellulose acetate membrane by means of gelatine which was then cross linked with glutaraldehyde. The systems were calibrated for different phenolic substances. Detection ranges were 0.1-1.0 μM for phenol and 0.05-1.0 μM for L-tyrosine and L-DOPA, respectively, with a response time of 3 min. Furthermore, phenol detection was performed in the presence of synthetic wastewater samples. © 2003 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1325 - 1329
Number of pages5
JournalProcess Biochemistry
Volume39
Issue number11
DOIs
Publication statusPublished - 30 Jul 2004

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Timur, S., Seta, L. D., Pazarlioǧlu, N., Pilloton, R., & Telefoncu, A. (2004). Screen printed graphite biosensors based on bacterial cells. Process Biochemistry, 39(11), 1325 - 1329. https://doi.org/10.1016/S0032-9592(03)00265-6