Selection of cell-lines and regeneration of plants resistant to fusaric acid from Gladiolus x grandiflorus cv. 'Peter Pears'

Patrizio C. Remotti, Hubertus J.M. Löffler, Lous Van Vloten-Doting

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Abstract

Cell suspension cultures of 'Peter Pears', a cultivar of Gladiolus x grandiflorus (Hort.), susceptible to the fungus Fusarium oxysporum f. sp. gladioli (Mass.), have been challenged with fusaric acid, one of the toxins produced by this pathogen. Selected cell-lines showed increased tolerance to the toxin and grew even on concentrations of fusaric acid up to 0.5 mM. When inoculated with a conidial suspension, the mycelial growth on selected cell-lines was limited compared to the control. Fusaric acid greatly reduced plant regeneration, and only two plants were obtained from the callus subcultured for a prolonged time on medium with fusaric acid. These plants have a significantly altered DNA content compared to the control. A reduced callus phase on toxin-containing medium, resulted in an improved regeneration and the DNA content of the regenerated plantlets was similar to the control. Some plants regenerated from these fusaric acid-insensitive cell-lines showed an increased tolerance to the toxin when cultured in vitro in presence of fusaric acid. The selected plants will be further tested for Fusarium-resistance once maturity is reached.
Original languageEnglish
Pages (from-to)237 - 245
Number of pages9
JournalEuphytica
Volume96
Issue number2
DOIs
Publication statusPublished - 1997
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

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