Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - A study of spouses of pregnant women in three geographical regions

Ina Olmer Specht, Karin S. Hougaard, Marcello Spanò, Davide Bizzaro, Gian Carlo Manicardi, Christian H. Lindh, Gunnar Toft, Bo A.G. Jönsson, Aleksander Giwercman, Jens Peter E. Bonde

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38 Citations (Scopus)


Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found. © 2012 Elsevier Inc.
Original languageEnglish
Pages (from-to)577 - 583
Number of pages7
JournalReproductive Toxicology
Issue number4
Publication statusPublished - Jul 2012
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Toxicology

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