Structural and catalytic properties and homology modelling of the human nucleoside diphosphate kinase C, product of the DRnm23 gene

Muriel Erent, Philippe Gonin, Jacqueline Cherfils, Pierre Tissier, Giuseppe Raschellà, Anna Giartosio, Fabrice Agou, Claude Sarger, Marie-Lise Lacombe, Manfred Konrad, Ioan Lascu

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21 Citations (Scopus)

Abstract

The human DRnm23 gene was identified by differential screening of a cDNA library obtained from chronic myeloid leukaemia-blast crisis primary cells. The over-expression of this gene inhibits differentiation and induces the apoptosis of myeloid precursor cell lines. We overproduced in bacteria a truncated form of the encoded protein lacking the first 17 N-terminal amino acids. This truncated protein was called nucleoside diphosphate (NDP) kinase CΔ. NDP kinase CΔ had similar kinetic properties to the major human NDP kinases A and B, but was significantly more stable to denaturation by urea and heat. Analysis of denaturation by urea, using size exclusion chromatography, indicated unfolding without the dissociation of subunits, whereas renaturation occurred via a folded monomer. The stability of the protein depended primarily on subunit interactions. Homology modelling of the structure of NDP kinase CΔ, based on the crystal structure of NDP kinase B, indicated that NDP kinase CΔ had several additional stabilizing interactions. The overall structure of the two enzymes appears to be identical because NDP kinase CΔ readily formed mixed hexamers with NDP kinase A. It is possible that mixed hexamers can be observed in vivo.
Original languageEnglish
Pages (from-to)1972 - 1981
Number of pages10
JournalEuropean Journal of Biochemistry
Volume268
Issue number7
DOIs
Publication statusPublished - 2001
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry

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