The RB-related gene Rb2/p130 in neuroblastoma dierentiation and in B-myb promoter down-regulation

Giuseppe Raschellà, Barbara Tanno, Francesco Bonetto, Anna Negroni, Pier Paolo Claudio, Alfonso Baldi, Roberto Amendola, Bruno Calabretta, Antonio Giordano, Marco G. Paggi

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27 Citations (Scopus)

Abstract

The retinoblastoma family of nuclear factors is composed of RB, the prototype of the tumour suppressor genes and of the strictly related genes p107 and Rb2/p130. The three genes code for proteins, namely pRb, p107 and pRb2/p130, that share similar structures and functions. These proteins are expressed, often simultaneously, in many cell types and are involved in the regulation of proliferation and differentiation. We determined the expression and the phosphorylation of the RB family gene products during the DMSO-induced differentiation of the N1E-115 murine neuroblastoma cells. In this system, pRb2/p130 was strongly up-regulated during mid-late differentiation stages, while, on the contrary, pRb and p107 resulted markedly decreased at late stages. Differentiating N1E-115 cells also showed a progressive decrease in B-myb levels, a proliferation-related protein whose constitutive expression inhibits neuronal differentiation. Transfection of each of the RB family genes in these cells was able, at different degrees, to induce neuronal differentiation, to inhibit [3H]thymidine incorporation and to down-regulate the activity of the B-myb promoter.
Original languageEnglish
Pages (from-to)401 - 407
Number of pages7
JournalCell Death and Differentiation
Volume5
Issue number5
DOIs
Publication statusPublished - 1998
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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