The starch-bound alpha-amylase/trypsin-inhibitors in Avena

Laura Gazza, Gloria Gazzelloni, Federica Taddei, Arianna Latini, Vera Muccilli, Michela Alfieri, Salvatore Conti, Rita Redaelli, Norberto E. Pogna

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Abstract

Oat kernels exhibit an extra-soft texture, a trait recently demonstrated to be largely modulated by starch-bound tryptophan-rich 2S proteins, the vromindolines. In this study, fractionation by two-dimensional electrophoresis of starch-bound proteins in 25 oat (Avena sativa) cultivars and 11 diploid or tetraploid Avena species revealed novel 2S proteins called Avena α-amylase/trypsin-inhibitors (AATI) because of their sequence similarity with wheat α-amylase/trypsin inhibitors. Thirty-seven AATI polypeptides, about 14 kDa in size, were split into three families named AATI-1, AATI-2, and AATI-3 with different primary structures and isoelectric points. AATI-1 and AATI-2 proteins showed 55.5–60.0 % sequence similarity with wheat α-amylase inhibitors CM1, CM2, and CM16, which have been found to cause innate immunity responses in celiac disease and non-celiac gluten sensitivity. Diploid A-genome and tetraploid AC-genome oat species possess three and five genes encoding for the AATI proteins, respectively, whereas hexaploid A. sativa exhibits 12 genes dispersed over the A-, C-, and D-genomes. Some AATI proteins expressed in hexaploid oats were assigned to the A-genome based on similarity to their counterparts in diploid species, contributing to further clarify the genetic origin of hexaploid oats. Moreover, AATI may interact with starch-bound vromindolines in determining the extra-soft texture of oat kernels and, due to their balanced amino acid compositions, may contribute to the biological value of oat proteins in a positive manner.
Original languageEnglish
Pages (from-to)2043 - 2054
Number of pages12
JournalMolecular Genetics and Genomics
Volume291
Issue number6
DOIs
Publication statusPublished - 1 Dec 2016
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Gazza, L., Gazzelloni, G., Taddei, F., Latini, A., Muccilli, V., Alfieri, M., ... Pogna, N. E. (2016). The starch-bound alpha-amylase/trypsin-inhibitors in Avena. Molecular Genetics and Genomics, 291(6), 2043 - 2054. https://doi.org/10.1007/s00438-016-1238-4