Purpose : Our goal was to identify genes showing a general transcriptional response to irradiation in mammalian cells and to analyze their response in function of dose, time and quality of irradiation and of cell type. Materials and methods : We used a modifi ed MIAME (Minimal Information About Microarray Experiments) protocol to import microarray data from 177 diff erent irradiation conditions in the Radiation Genes database and performed cut-off-based selections and hierarchical gene clustering. Results : We identifi ed a set of 29 genes which respond to a wide range of irradiation conditions in diff erent cell types and tissues. Functional analysis of the negatively modulated genes revealed a dominant signature of mitotic cell cycle regulation which appears both dose and time-dependent. This signature is prominent in cancer cells and highly proliferating tissues but it is strongly attenuated in non cancer cells. Conclusions : The transcriptional response of mammalian cancer cells to irradiation is dominated by a mitotic cell cycle signature both dose and time-dependent. This core response, which is present in cancer cells and highly proliferating tissues such as skin, blood and lymph node, is weaker or absent in non-cancer cells and in liver and spleen. CDKN1A (cyclin-dependent kinase inhibitor 1A) appears as the most generally induced mammalian gene and its response (mostly dose-and time-independent) seems to go beyond the typical DNA damage response. © 2012 Informa UK, Ltd.
All Science Journal Classification (ASJC) codes
- Radiological and Ultrasound Technology
- Radiology Nuclear Medicine and imaging
Bufalieri, F., Licursi, V., D'Antonio, M., Castrignanò, T., Amendola, R., & Negri, R. (2012). The transcriptional response of mammalian cancer cells to irradiation is dominated by a cell cycle signature which is strongly attenuated in non-cancer cells and tissues. International Journal of Radiation Biology, 88(11), 822 - 829. https://doi.org/10.3109/09553002.2012.676230