The transcriptional response of mammalian cancer cells to irradiation is dominated by a cell cycle signature which is strongly attenuated in non-cancer cells and tissues

Francesca Bufalieri, Valerio Licursi, Mattia D'Antonio, Tiziana Castrignanò, Roberto Amendola, Rodolfo Negri

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose : Our goal was to identify genes showing a general transcriptional response to irradiation in mammalian cells and to analyze their response in function of dose, time and quality of irradiation and of cell type. Materials and methods : We used a modifi ed MIAME (Minimal Information About Microarray Experiments) protocol to import microarray data from 177 diff erent irradiation conditions in the Radiation Genes database and performed cut-off-based selections and hierarchical gene clustering. Results : We identifi ed a set of 29 genes which respond to a wide range of irradiation conditions in diff erent cell types and tissues. Functional analysis of the negatively modulated genes revealed a dominant signature of mitotic cell cycle regulation which appears both dose and time-dependent. This signature is prominent in cancer cells and highly proliferating tissues but it is strongly attenuated in non cancer cells. Conclusions : The transcriptional response of mammalian cancer cells to irradiation is dominated by a mitotic cell cycle signature both dose and time-dependent. This core response, which is present in cancer cells and highly proliferating tissues such as skin, blood and lymph node, is weaker or absent in non-cancer cells and in liver and spleen. CDKN1A (cyclin-dependent kinase inhibitor 1A) appears as the most generally induced mammalian gene and its response (mostly dose-and time-independent) seems to go beyond the typical DNA damage response. © 2012 Informa UK, Ltd.
Original languageEnglish
Pages (from-to)822 - 829
Number of pages8
JournalInternational Journal of Radiation Biology
Volume88
Issue number11
DOIs
Publication statusPublished - Nov 2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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