Thick film sensors based on laccases from different sources immobilized in polyaniline matrix

Suna Timur, Nurdan Pazarloǧlu, Roberto Pilloton, Azmi Telefoncu

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Abstract

Thick film electrode based biosensors containing Trametes versicolor (TvL) and Aspergillus niger (AnL) laccases and Agaricus bisporus tissues (AbT) were developed for the determination of phenolic compounds and the measurement was based on oxygen consumption in relation to analyte oxidation. The electrodeposited organic polymer; polyaniline was used as a matrix for the immobilization in the preparation of thick film sensors. The systems were calibrated for different phenolic substances. A linearity was obtained in concentration range between 0.4 and 6.0μM phenol, 0.2 and 1.0μM catechol, 2.0 and 20.0μM L-DOPA for TvL based biosensor; for AnL based enzyme electrode 0.4 and 4.0μM phenol, 0.4 and 15μM catechol, 0.4 and 6.0μM L-DOPA; for AbT electrode 1.0 and 10μM phenol, 0.4 and 1.6μM catechol, 1. 0 and 10μM L-DOPA, respectively, in the response time of 300s. Furthermore, as well as sample application and accuracy, optimum pH, temperature and thermal stabilities of the proposed systems were also detected. © 2003 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)132 - 136
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume97
Issue number1
DOIs
Publication statusPublished - 1 Jan 2004
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

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