Time-resolved absorption as optical method for herbicide detection

Francesco Baldini, Claudio Domenici, Ambra Gianneti, Domenica Masci, A. Mencaglia

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12 Citations (Scopus)


A new sensing system for the detection of photosynthetic herbicides in water has been developed, based on the use of a trans-membrane protein complex, the reaction centre (RC) isolated from Rhodobacter sphaeroides. The stationary and excited state of this protein are characterised by different absorption properties. The path followed by the protein to return to the stationary state is influenced by the presence of photosynthetic herbicides. Therefore the concentration of herbicides could be measured by monitoring the temporal changes of absorption following optical excitation. For this purpose, an optoelectronic system has been realised. It makes use of a 860 nm light emitting diode and a hybrid photodetector and is coupled to a 5 cm-long optical cell containing the RC solution through optical fibres. The system was tested with atrazine and a limit of detection of 10 nM was obtained. © 2003 Elsevier Science B.V. All rights reserved.
Original languageEnglish
Pages (from-to)198 - 203
Number of pages6
JournalSensors and Actuators, B: Chemical
Issue number1-3
Publication statusPublished - 20 Apr 2003
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry
  • Electrical and Electronic Engineering

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