Toxicity of toner nanoparticles on RT112 cell cultures

Lucia Mosiello, Giovanna Zappa, Claudia Zoani, Ilaria Lamberti, Rosanna Gatti, Luciano Pilloni

Research output: Contribution to conferencePaper

Abstract

The measurement of cell proliferation and cell viability has become a key technology in the life sciences. The need for sensitive, reliable, fast and easy methods has led to the development of several standard assays. These include the determination of DNA synthesis by measuring the amount of radioactive labeled nucleosides like [3H]-thymidine incorporated in nucleic acid. Alternatively several tetrazolium salts, including MTT, XTT and MTS are usually used to assay cell proliferation and viability on microplate assays. Actually there is an immediate need for nanotoxicology the availability of rapid and inexpensive tools in order to carry out some preliminary test for nanomaterials and the aim of our work was to investigate on the feasibility of WST-1 assay for this purpose. In our aim we investigated the toxicity in term of cell proliferation of toner nanoparticles (5-50 nm) on human bladder carcinoma cell line RT112 by WST-1 assay and also to evaluate the feasibility to apply this method as tool for in vitro nanotoxicology studies. In order to relate the toxicity test results to the toner powder properties, chemical and physical-chemical analyses have been performed in parallel on the same batch sample. © 2009 IEEE NANO Organizers.
Original languageEnglish
Publication statusPublished - 2009
Externally publishedYes
Event2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009 - , Italy
Duration: 1 Jan 2009 → …

Conference

Conference2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009
CountryItaly
Period1/1/09 → …

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Process Chemistry and Technology
  • Electrical and Electronic Engineering

Cite this

Mosiello, L., Zappa, G., Zoani, C., Lamberti, I., Gatti, R., & Pilloni, L. (2009). Toxicity of toner nanoparticles on RT112 cell cultures. Paper presented at 2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009, Italy.