Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack

Paraskevi Tavladoraki, Eugenio Benvenuto, Stefania Trinca, Domenico De Martinis, Antonino Cattaneo, Patrizia Galeffi

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Abstract

EXPRESSION of viral genes in transgenic plants is a very effective tool for attenuating plant viral infection1-3. Nevertheless, the lack of generality and risk issues related to the expression of viral genes in plants4might limit the exploitation of this strategy. Expression in plants of antibodies against essential viral proteins could provide an alternative approach to engineer viral resistance. Recently, expression of complete5-7or engineered7-9antibodies has been successfully achieved in plants. The engineered single-chain Fv antbody scFv (refs 10,11) is particularly suitable for expression in plants because of its small size and the lack of assembly requirements. Here we present evidence that constitutive expression in transgenic plants of a scFv antibody, directed against the plant icosahedral tombusvirus artichoke mottled crinkle virus, causes reduction of infection incidence and delay in symptom development. © 1993 Nature Publishing Group.
Original languageEnglish
Pages (from-to)469 - 472
Number of pages4
JournalNature
Volume366
Issue number6454
DOIs
Publication statusPublished - 1993
Externally publishedYes

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