Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase μ isoform

Marzia Bianchi, Roberto Amendola, Rodolfo Federico, Fabio Polticelli, Paolo Mariottini

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17 Citations (Scopus)

Abstract

In mouse, at least two catalytically active splice variants (mSMOα and mSMOμ) of the flavin-containing spermine oxidase enzyme are present. We have demonstrated previously that the cytosolic mSMOα is the major isoform, while the mSMOμ enzyme is present in both nuclear and cytoplasmic compartments and has an extra protein domain corresponding to the additional exon VIa. By amino acid sequence comparison and molecular modeling of mSMO proteins, we identified a second domain that is necessary for nuclear localization of the mSMOμ splice variant. A deletion mutant enzyme of this region was constructed to demonstrate its role in protein nuclear targeting by means of transient expression in the murine neuroblastoma cell line, N18TG2. © 2005 FEBS.
Original languageEnglish
Pages (from-to)3052 - 3059
Number of pages8
JournalFEBS Journal
Volume272
Issue number12
DOIs
Publication statusPublished - Jun 2005
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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