Use of the gyrB gene to discriminate among species of the Burkholderia cepacia complex

Silvia Tabacchioni, Lorenzo Ferri, Graziana Manno, Massimo Mentasti, Priscilla Cocchi, Silvia Campana, Novella Ravenni, Giovanni Taccetti, Claudia Dalmastri, Luigi Chiarini, Annamaria Bevivino, Renato Fani

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Abstract

Bacteria of the Burkholderia cepacia complex (Bcc) are opportunistic pathogens that can cause serious infections in lungs of cystic fibrosis patients. The Bcc comprises at least nine species that have been discriminated by a polyphasic taxonomic approach. In this study, we focused on the gyrB gene, universally distributed among bacteria, as a new target gene to discriminate among the Bcc species. New PCR primers were designed to amplify a gyrB DNA fragment of about 1900 bp from 76 strains representative of all Bcc species. Nucleotide sequences of PCR products were determined and showed more than 400 polymorphic sites with high sequence similarity values from most isolates of the same species. Phylogenetic tree analysis revealed that most of the 76 gyrB sequences grouped, forming clusters, each corresponding to a given Bcc species. © 2008 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd.
Original languageEnglish
Pages (from-to)175 - 182
Number of pages8
JournalFEMS Microbiology Letters
Volume281
Issue number2
DOIs
Publication statusPublished - Apr 2008
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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