Soft X-ray contact microscopy (SXCM), using a pulsed X-ray source, offers the possibility of imaging the ultrastructure of living biological systems at sub-100 nm resolution. We have developed a table-top pulsed plasma X-ray source for this application, generated by a large-volume XeCI laser, achieving a good conversion efficiency to 'water-window' X-rays (hv ≃ 280-530 eV). Optimum plasma conditions for SXCM are discussed, including the effect of pulse duration, target material and resist development time on image resolution. Soft X-ray contact images of Chlamydomonas dysosmos (unicellular alga) and of the cyanobacterium Leptolyngbya are shown.
|Pages (from-to)||96 - 103|
|Number of pages||8|
|Journal||Journal of Microscopy|
|Publication status||Published - 1997|
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
Albertano, P., Reale, L., Palladino, L., Reale, A., Cotton, R., Bollanti, S., ... Grilli, A. (1997). X-ray contact microscopy using an excimer laser plasma source with different target materials and laser pulse durations. Journal of Microscopy, 187(2), 96 - 103. https://doi.org/10.1046/j.1365-2818.1997.2120768.x